Apoptosis Methods in Pharmacology and Toxicology: Approaches - download pdf or read online
By Myrtle A. Davis
Dr. Myrtle A. Davis has assembled a panel of state-of-the-art scientists to explain their most sensible tools for detecting, illuminating, and quantifying apoptotic mechanisms in a fashion that's precious for the layout of toxicology and pharmacology reviews. those cutting-edge strategies comprise circulation cytometric, fluorometric, and laser scanning equipment for quantifying and characterizing apoptosis, in addition to protocols for using DNA microarray know-how, excessive throughput displays, and ELISA. Immunocytochemical tools for measuring biochemical and molecular endpoints in tissue sections might be hugely beneficial for these conducting reports in entire animal types instead of phone tradition structures.
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Additional info for Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification (Methods in Pharmacology and Toxicology)
These dyes have been successfully used in multiparametric analyses, using both flow cytometry and confocal microscopy, which has led to an enhanced understanding of the role of [Ca2+]i in apoptosis. Burchiel et al. (51) have recently published a review of multiparametric flow cytometric Ca2+ analysis. Because flow cytometry measures total cellular fluorescence, most flow cytometric methods of assessing total [Ca 2+]i may not be sensitive to apoptosis-induced redistribution of [Ca2+]i. In 32D cells induced to undergo apoptosis through interleukin-3 (IL-3) withdrawal, total [Ca2+]i remained unaltered, although a significant subcellular redistribution of Ca2+ from the cytoplasm to the mitochondria occurred, which could be inhibited by Bcl-2 expression (52).
Necrapoptosis and the mitochondrial permeability transition: shared pathways to necrosis and apoptosis. Am. J. Physiol. 276(1 Pt 1), G1–G6. 4. Warny, M. and Kelly, C. P. (1999) Monocytic cell necrosis is mediated by potassium depletion and caspase-like proteases. Am. J. Physiol. 276(3 Pt 1), C717–C724. 5. Raffray, M. and Cohen, G. M. (1997) Apoptosis and necrosis in toxicology: a continuum or distinct modes of cell death? Pharmacol. Ther. 75(3), 153–177. 6. Cotran, R. , and Robbins, S. L. (1999) Robbin’s Pathological Basis of Disease.
Simpson, A. , and Pozzan, T. (1992) Rapid changes of mitochondrial Ca2+ revealed by specifically targeted recombinant aequorin [published erratum appears in Nature 1992 Dec 24–31; 360(6406):768]. Nature 358(6384), 325–327. 50. ) (1994) A Practical Guide to the Study of Calcium in Living Cells. Academic Press, San Diego. 51. Burchiel, S. , Edwards, B. , Kuckuck, F. , Lauer, F. , Prossnitz, E. , Ransom, J. , and Sklar, L. A. (2000) Analysis of free intracellular calcium by flow cytometry: multiparameter and pharmacologic applications.
Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification (Methods in Pharmacology and Toxicology) by Myrtle A. Davis